| Assay no | Test | Verbal result within |
|---|
| BT 201 | XC Plaque Assay
- Detection of infectious murine ecotropic retroviruses
- Confirmation of borderline results by means of an immunoassay | 2 weeks |
| BT 202 | S+L- Focus Assay
- Detection of infectious murine xenotropic retroviruses
- Confirmation of borderline results by means of an immunoassay | 2 weeks |
| BT 203 | Reverse Transcriptase Assay
- Assay of pellets in presence of Mg++ or Mn++ to detect human, murine and other retroviruses
- Concurrent positive and negative controls
- Confirmation of a positive result is performed by repetition of the assay with two different template-primers in order to discriminate Reverse Transcriptase from cellular DNA polymerase | 2 weeks |
| BT 204 | XC Plaque Extended Assay
- Subculture with murine detector cells to amplify contaminating virus prior to Assay #BT201 | 5 weeks |
| BT 205 | S+L- Focus Assay
- Subculture with murine detector cells to amplify contaminating virus prior to Assay #BT202 | 6 weeks |
| BT 206 | Mink Cell Focus Assay
- Detection of infectious polytropic murine retroviruses | 3 weeks |
| BT 207 | Mink Cell Focus Extended Assay
- Subculture with mink detector cells to amplify contaminating virus prior to Assay #BT202 | 5 weeks |
| BT 208 | Co-cultivation Assay with Mus Dunii Cells
- Test is performed in compliance with FDA 'Points to consider'
- Co-cultivation of test cells with Mus Dunni cells for 5 passages
- At passage 1 and 5 the cell culture supernatant is tested by means of XC Plaque Assay (#BT 201) and S+L- Focus Assay (#BT202) | 5 weeks |
| BT 210 | Transmission Electron Microscopy (qualitative)
- Detection of viruses in cell cultures by means of examination of 100 cells | 8 weeks |
| BT 211 | Transmission Electron Microscopy (quantitative)
- Detection and quantification of viruses in cell cultures by means of examination of 200 cells | 8 weeks |
| BT 212 | Retrovirus Detection and Quantitation by Negative Stain Electron Microscopy
- Detection and quantitation of typical retroviral particles | 8 weeks |
